Guido Messina (4 January 1931 – 10 January 2020) was an Italian road and track cyclist.

He was born in Monreale, Italy, on 4 January 1931. On track he won five world titles in the individual 4000 m pursuit between 1948 and 1956, and a gold medal with the Italian team at the 1952 Olympics (individual pursuit became an Olympic event only in 1964, when Messina retired from cycling). Between 1954 and 1963 he rode professionally and won the first stage of the 1955 Giro d'Italia. He died six days after his 89th birthday on 10 January 2020.