Jonathan Breyne (born 4 January 1991) is a Belgian cyclist, who currently rides for Belgian amateur team Gaverzicht–BE Okay.

In 2013, Breyne was accused of using illegal drugs following the discovery of clenbuterol in a sample taken following his participation in the Japan Cup. Breyne was cleared by the Union Cycliste Internationale, who accepted the drug had entered his system as a result of eating contaminated meat while taking part in a race in China.

In 2016, Breyne signed a contract with the Belgian continental team Tarteletto–Isorex.